Skip to main content

Must

Study of electrocardiogram

  Electrocardiogram   The record of electrical events occurring during intiation and spread of the kar liya action potentials is the electro cardiogram the instrument used to record the changes of wave is the electrocardiogram electrocardiogram the study of electrical events being with work of kolekar and malaria while working in the amphibian heart first complete recording of made by in 1887.  The sequence of waves recorded from mammalian heart include the p waves your waves and the table the first wave or to the p wave is a small effort change it represents the diploma reason of the attriya the spread of an impulse from the essay notes through the muscles of the attriya in a contribution at the velocity of about 0.3 MS fraction of 2nd after downward. Tea polarization sins the attriya are connected electrically to the ventricles only through the avenout the expression spreads to the ventricles through small tongs Nil fibres in with the velocity of the wave of exhibition ...
Post a Comment
Read more

To study of morphology,vectoship,pathogenicity, and control of mosquito.anaphelrs,culex, and,aedes.

 Aim:-To study of morphology,vectoship,pathogenicity, and control of mosquito.anaphelrs,culex, and,aedes. Morphology of mosquito:- Body is divided into head,thorax and abdomen. Head:- Head is globular and highly modified on a cylinder next rare there are two large black compound ice without oceally the head bears to maximum reforms and a proper mouth parts are seeking persing type.  Thorax:- Thorax is arched and it has meso thorax which is very nice and it's tagem has three calories and captain and a trilode captain and sports ketam project sex method methods are reduced to form a small to paints of spiricals are present. Abdomen:- Abdomen consider of a 10 segments of page which the first westigial 28 are clearly seen each has a pair of second to 8 are spiral the segments has a biode dorsal steam and with project posteriorly.  Piercing& sucking mouth of mosquito:- The labium is modified two form a long straight Plassey tube cost probies it has diployed Guru on its upp...
Post a Comment

To study the preparation of permanent slide by micro technique using microtome.

 Aim:-To study the preparation of permanent slide by micro technique using microtome.


Requirements:-coupling jar , reagent bottles,slide,coverglass,spirit,puraffin wax,beaker,l-blocks,lamp, microtome,blade,wooden block.


General outline:-

Animal tissue for microtome ->necrotizatio of animals ->fixing of tissues ->washing ->dehydrotion->claring or dealcoholization of tissue ->Embedding of tissue ->block making ->trimming ->section cutting ->double staining, dehydration, clearing and mountings ->microscopic study ->microscopic photography.


Procedure:-


Animals aur tissue for microtone normally frog rat black red are favourable for microsome is in vertebrate animals file in invertebrate earthworm is used.


Necro Jason of animals generally chloroform is used to nephrotic necrotise animal take a big jar and make a cotton bed on bottom of there bottom of jar 


Sprinkle the chloroform on the cotton and put animal in the year in the jar 


After 15 to 20 times when animal fully entities take out the animal and keep it in desceting frog and asect the animal 


Fixing of tissue tissue is generally fixing in the bounds floats C for 100 ml saturated picnic acid 75 ml floram 20 ml glycerine acetic acid 5 ml 


Other pics you like acetone formalin methanol also can be used 


Washing of tissue after the fixation of 24 hours take out the tissue from the fixit and keep it under slow running tape water for 24 hours 


The induction of complete removal of picnic acid comes when no yellow is water is seen 


Dehydration for the dehydration of tissue one should have to pass through alcohol series 




The alcoholsation or clearing of tissue normally the clearing agents are the xylene and cdwood oil first keep the tissue in solution which content region one alcohol xylem ratio the solution release by one M1 alcohol xylem ratio 


Embedding wax is used for embeding tissue take a tissue from xylene and keep it in a bigger containing Island Max 4 30 minutes then transfer that issue in methyl wax for ambouring for 1 to 2 hours 


Block making make the block either in the metal elsept angle or in paper boat or cavity before block making apply glycerine on internal surface of the blocks poor the melted wax at the bottom of refrigerator cavity formed by l block at tissue and feather and more melted wax over the tissue to the cover during block making take a care of out of air bubbles 


Dreaming dream the wax around the embedded material and the make a permanent triangular block on the one side keep sufficient space of the block of a fixing on the black holder 


Section cutting the blocks are cut either by rocking microtome or rotary microsome at the six micro thickness ribbon are Cape over measures arbi mean cottage night keep the slide ready apply pin had had mayor beam in over the right and the rabbit by last finger where in the speaking in the section over glass surface give to three rows of section depending up on the size of the section 


Double staining the hydration clearing mounting double staining is applied hemosis and is in nucleus and cytosin of the cell repres in take the individual slide and keep it in dialling to remove x45 to 10 minutes now wax is removed from the xylem tissue is free now.

Aim:-To study the preparation of permanent slide by micro technique using microtome.




Requirements:-coupling jar , reagent bottles,slide,coverglass,spirit,puraffin wax,beaker,l-blocks,lamp, microtome,blade,wooden block.




General outline:-


Animal tissue for microtome ->necrotizatio of animals ->fixing of tissues ->washing ->dehydrotion->claring or dealcoholization of tissue ->Embedding of tissue ->block making ->trimming ->section cutting ->double staining, dehydration, clearing and mountings ->microscopic study ->microscopic photography.




Procedure:-




Animals aur tissue for microtone normally frog rat black red are favourable for microsome is in vertebrate animals file in invertebrate earthworm is used.




Necro Jason of animals generally chloroform is used to nephrotic necrotise animal take a big jar and make a cotton bed on bottom of there bottom of jar 




Sprinkle the chloroform on the cotton and put animal in the year in the jar 




After 15 to 20 times when animal fully entities take out the animal and keep it in desceting frog and asect the animal 




Fixing of tissue tissue is generally fixing in the bounds floats C for 100 ml saturated picnic acid 75 ml floram 20 ml glycerine acetic acid 5 ml 




Other pics you like acetone formalin methanol also can be used 




Washing of tissue after the fixation of 24 hours take out the tissue from the fixit and keep it under slow running tape water for 24 hours 




The induction of complete removal of picnic acid comes when no yellow is water is seen 




Dehydration for the dehydration of tissue one should have to pass through alcohol series 








The alcoholsation or clearing of tissue normally the clearing agents are the xylene and cdwood oil first keep the tissue in solution which content region one alcohol xylem ratio the solution release by one M1 alcohol xylem ratio 




Embedding wax is used for embeding tissue take a tissue from xylene and keep it in a bigger containing Island Max 4 30 minutes then transfer that issue in methyl wax for ambouring for 1 to 2 hours 




Block making make the block either in the metal elsept angle or in paper boat or cavity before block making apply glycerine on internal surface of the blocks poor the melted wax at the bottom of refrigerator cavity formed by l block at tissue and feather and more melted wax over the tissue to the cover during block making take a care of out of air bubbles 




Dreaming dream the wax around the embedded material and the make a permanent triangular block on the one side keep sufficient space of the block of a fixing on the black holder 




Section cutting the blocks are cut either by rocking microtome or rotary microsome at the six micro thickness ribbon are Cape over measures arbi mean cottage night keep the slide ready apply pin had had mayor beam in over the right and the rabbit by last finger where in the speaking in the section over glass surface give to three rows of section depending up on the size of the section 




Double staining the hydration clearing mounting double staining is applied hemosis and is in nucleus and cytosin of the cell repres in take the individual slide and keep it in dialling to remove x45 to 10 minutes now wax is removed from the xylem tissue is free now.

Labels:
Location: India

Comments

Post a Comment

Popular posts from this blog

To study of total RBC count in human blood.

  To study of total RBC count in human blood . Apparatus: Haemocytometer,sterilized prcking needle,r.b.c pipette, compound microscope and hayems diluting fluid ( Nacl-1%,na2so4-2.5%,and Hgcl2) #Haemocytometer   =Haemocytometer is a kind of slide containing counting chambers. =The Burker, Neubaur,thoma or any other Haemocytometer may be used. =The Neubaur Haemocytometer consists of a double -cell slide with two sunk platforms in open cell type. =Each platform has a rulling so that rapid duplicate counts can be made. =When a special cover glass is kept in position,a depth of 1/10 mm is maintained over the rulligs. =Diluted blood Is run on by capillary force after the cover glass has been fixed. =Gunting chambers are nine large square and each medium sides of1mm each. =Central large square ABCD is subdivided in 25 medium squares and each medium in turn contain 16 tiny squares having sides 0.05 mm long. =Hayens diluting fluid may be kept as stock solution and a small amount of it ...
Post a Comment
Read more

Study of electrocardiogram

  Electrocardiogram   The record of electrical events occurring during intiation and spread of the kar liya action potentials is the electro cardiogram the instrument used to record the changes of wave is the electrocardiogram electrocardiogram the study of electrical events being with work of kolekar and malaria while working in the amphibian heart first complete recording of made by in 1887.  The sequence of waves recorded from mammalian heart include the p waves your waves and the table the first wave or to the p wave is a small effort change it represents the diploma reason of the attriya the spread of an impulse from the essay notes through the muscles of the attriya in a contribution at the velocity of about 0.3 MS fraction of 2nd after downward. Tea polarization sins the attriya are connected electrically to the ventricles only through the avenout the expression spreads to the ventricles through small tongs Nil fibres in with the velocity of the wave of exhibition ...
Post a Comment
Read more