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To study the preparation of permanent slide by micro technique using microtome.

  Aim:- To study the preparation of permanent slide by micro technique using microtome. Requirements:- coupling jar , reagent bottles,slide,coverglass,spirit,puraffin wax,beaker,l-blocks,lamp, microtome,blade,wooden block. General outline:- Animal tissue for microtome ->necrotizatio of animals ->fixing of tissues ->washing ->dehydrotion->claring or dealcoholization of tissue ->Embedding of tissue ->block making ->trimming ->section cutting ->double staining, dehydration, clearing and mountings ->microscopic study ->microscopic photography. Procedure:- Animals aur tissue for microtone normally frog rat black red are favourable for microsome is in vertebrate animals file in invertebrate earthworm is used. Necro Jason of animals generally chloroform is used to nephrotic necrotise animal take a big jar and make a cotton bed on bottom of there bottom of jar  Sprinkle the chloroform on the cotton and put animal in the year in the jar  After 1...
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To study of morphology,vectoship,pathogenicity, and control of mosquito.anaphelrs,culex, and,aedes.

 Aim:-To study of morphology,vectoship,pathogenicity, and control of mosquito.anaphelrs,culex, and,aedes. Morphology of mosquito:- Body is divided into head,thorax and abdomen. Head:- Head is globular and highly modified on a cylinder next rare there are two large black compound ice without oceally the head bears to maximum reforms and a proper mouth parts are seeking persing type.  Thorax:- Thorax is arched and it has meso thorax which is very nice and it's tagem has three calories and captain and a trilode captain and sports ketam project sex method methods are reduced to form a small to paints of spiricals are present. Abdomen:- Abdomen consider of a 10 segments of page which the first westigial 28 are clearly seen each has a pair of second to 8 are spiral the segments has a biode dorsal steam and with project posteriorly.  Piercing& sucking mouth of mosquito:- The labium is modified two form a long straight Plassey tube cost probies it has diployed Guru on its upp...
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To study permanent slides of meosis.

 To study permanent slides of meosis.

Meosis-1 

Prophase -01

It is of long duration and differs from prophes of mitosis. It is further divided into the following sun stages.

Leptotene:-

The nucleus increase is in size and volume by absorbing water.

The chromatin network shows condensation and forms a number of long thread like structures called as chromosome.

Each chromosome show characteristics beaded appearençe due to presence of chromosomes along the entire length.

Nuclear membrane and nucleus are distinct.


Zygotene:-

The two homologous chromosomes lie side by side .

This is known as pairing or synopsis of homologous chromosomes.

Each pair of chromosomes known as bivalent.

Their in further sorting of chromosomes by coiling and thus they become more distinct.


Pachytene:-

Each chromosome of a buy valence please 

Split longitudely into two sister comedy show that by when and become to a treat. 


The two chromate are from east by valen partically coiled around each other to exchange their genetic material this is known as crossing over. 

The point of crossing occurs as a chest Mata.


Diplotene:-

Homologous chromosomes WeChat paird during zigot now start separating from the one of the one another .

As the chromosomes are showing gradual condension to show that their is a tendency that committed find to sleep out of the chromosomes. 

This is known as termination of cristimata. 

Chromosomes that staperative out but the separating is not complete. 

Nuclear membrane and nucleus are degenerating.


Dikinesis:-


The bivalents condense further and get and only due east to besan in the cytoism 

The separation of bad chromosome is almost complete 

Termination of chromoplast almost complete 

Nuclear membrane and nucleus disappear 


Metaphase -1


The bivalance come to die in the equatorial plane in such a way that one member faces one pole of the spindle apparatus and the other member to the outer Paul does the two membrane of the pair line on opposite side of equator. 

Nuclear membrane and nucleus completely disappear 

Centro near of chromosome are attached to the spindle fibres and phases towards their respective call .

Chromotids are aligned on equatorial palote.



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To study the preparation of permanent slide by micro technique using microtome.

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